Overview

  • Product name
  • Description
    Rabbit polyclonal to MEK6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-334 of Human MEK6 (Uniprot ID: P52564).

  • Positive control
    • 293T, HeLa, HepG2 and MOLT4 whole cell lysates; HeLa cells; Human N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
  • Tissue specificity
    Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Weakly autophosphorylated.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Form
    It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
  • Alternative names
    • Dual specificity mitogen activated protein kinase kinase 6 antibody
    • Dual specificity mitogen-activated protein kinase kinase 6 antibody
    • MAP kinase kinase 6 antibody
    • MAP2K6 antibody
    • MAPK/ERK kinase 6 antibody
    • MAPKK 6 antibody
    • MAPKK6 antibody
    • MEK 6 antibody
    • MEK6 antibody
    • Mitogen Activated Protein Kinase Kinase 6 antibody
    • MKK 6 antibody
    • MKK6 antibody
    • MP2K6_HUMAN antibody
    • PRKMK6 antibody
    • Protein kinase mitogen activated kinase 6 antibody
    • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
    • SAPK kinase 3 antibody
    • SAPKK-3 antibody
    • SAPKK3 antibody
    • SKK3 antibody
    • Stress-activated protein kinase kinase 3 antibody
    see all

Images

  • Anti-MEK6 antibody (ab154901) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling MEK6 with ab154901 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human N87 xenograft tissue labeling MEK6 with ab154901 at 1/500 dilution.

References

ab154901 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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