Overview

  • Product name
    Anti-MEK6 antibody [EP557Y]
    See all MEK6 primary antibodies
  • Description
    Rabbit monoclonal [EP557Y] to MEK6
  • Specificity
    ab33866 recognises MKK6.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the N terminal of human MKK6.

  • Positive control
    • Hela cell lysate
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab33866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • Function
      Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
    • Tissue specificity
      Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Weakly autophosphorylated.
      Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • Database links
    • Form
      It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
    • Alternative names
      • Dual specificity mitogen activated protein kinase kinase 6 antibody
      • Dual specificity mitogen-activated protein kinase kinase 6 antibody
      • MAP kinase kinase 6 antibody
      • MAP2K6 antibody
      • MAPK/ERK kinase 6 antibody
      • MAPKK 6 antibody
      • MAPKK6 antibody
      • MEK 6 antibody
      • MEK6 antibody
      • Mitogen Activated Protein Kinase Kinase 6 antibody
      • MKK 6 antibody
      • MKK6 antibody
      • MP2K6_HUMAN antibody
      • PRKMK6 antibody
      • Protein kinase mitogen activated kinase 6 antibody
      • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
      • SAPK kinase 3 antibody
      • SAPKK-3 antibody
      • SAPKK3 antibody
      • SKK3 antibody
      • Stress-activated protein kinase kinase 3 antibody
      see all

    Images



    • Predicted band size : 37 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
      Lanes 4, 8 and 12: A431 cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-MEK6 antibody [EP557Y] (ab33866) at 1/10000 dilution + Hela cell lysate

      Predicted band size : 37 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    References

    ab33866 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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