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Product name Anti-MEK6 antibody [EP557Y] See all MEK6 primary antibodies
Rabbit monoclonal [EP557Y] to MEK6
Specificity ab33866 recognises MKK6.
Tested applications Suitable for:
WB, IP more details Unsuitable for:
Flow Cyt,ICC or IHC
Mouse, Rat, Human
A synthetic peptide corresponding to residues in the N terminal of human MKK6.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration information loading...
Purity IgG fraction
Clone number EP557Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Application notes Is unsuitable for Flow Cyt,ICC or IHC.
Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Tissue specificity Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Sequence similarities Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modifications Weakly autophosphorylated. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Information by UniProt
Form It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
Dual specificity mitogen activated protein kinase kinase 6 antibody
Dual specificity mitogen-activated protein kinase kinase 6 antibody
MAP kinase kinase 6 antibody
Anti-MEK6 antibody [EP557Y] images
Western blot - Anti-MEK6 antibody [EP557Y] (ab33866)
Predicted band size : 37 kDa Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg) Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg) Lanes 3, 7 and 11: HeLa cell lysate (20 µg) Lanes 4, 8 and 12: A431 cell lysate (20 µg) Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and
ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - MKK6 antibody [EP557Y] (ab33866)
References for Anti-MEK6 antibody [EP557Y] (ab33866)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"