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Immunohistochemistry (Paraffin-embedded sections) - MKK6 (phospho S207) antibody (ab36722)
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Western blot - MKK6 (phospho S207) antibody (ab36722)
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Immunocytochemistry/ Immunofluorescence-MEK6 (phospho S207) antibody(ab36722)
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Product Name
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MEK6 (phospho S207) antibody
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See all MEK6 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MEK6 (phospho S207)
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Immunogen
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Synthetic phosphopeptide derived from human MKK6 around the phosphorylation site of Serine 207 (V-D-SP-V-A).
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody detects endogenous levels of MKK6 only when phosphorylated at Serine207.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma tissue and MDA-MB-435 cell extracts.
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Relevance
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Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.
As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEK6 (phospho S207) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MEK6 (phospho S207) antibody images, references, products related to ab36722 and other tools.
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MEK6 (phospho S207) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - MKK6 (phospho S207) antibody (ab36722)
Immunohistochemical analysis of paraffin embedded
breast carcinoma, using ab36772 diluted 1:50.
Left: Untreated Ab; Right: Ab pre-treated with synthesized phosphopeptide.
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Western blot - MKK6 (phospho S207) antibody (ab36722)
All lanes : MEK6 (phospho S207) antibody (ab36722) at 1/500 dilution
Lane 1 : Extracts from untreated MDA-MB-435 cells
Lane 2 : Extracts from UV treated MDA-MB-435 cells
Lysates/proteins at 30 µg per lane.
Predicted band size : 37 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
UV treatment protocol:
a. For adherent cells, cells were plated on 100-mm culture dishes with growth medium (basic medium containing 10% serum).
b. When the cells were at the log phase and 80-90% confluent, the culture media was drained. Cells were washed with fresh basic media (no serum) once.
c. Add 3ml fresh basic media per dish before irradiation.
d. Culture dishes were uncovered in a UV cross-linker. UV irradiation was carried out with 100 J/m2 (or with various other dosages).
e. Cells can be directly used to prepare cell lysis. If needed to reculture in some experiments, 3ml growth medium was added to per dish, and the cells were incubated at 37°C for 1~2 hours in a CO2 incubator.
For suspension cells, cells were collected by centrifugation (1000g, 10min) from cell supernatant from flasks. Resuspend the cells in 3ml of fresh basic media, and plate on a 100-mm plate for irradiation.
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Immunocytochemistry/ Immunofluorescence-MEK6 (phospho S207) antibody(ab36722)
ICC/IF image of ab36722 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36722, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to MEK6
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