Overview

  • Product nameAnti-MEK7 antibody [EP1455Y]
    See all MEK7 primary antibodies
  • Description
    Rabbit monoclonal [EP1455Y] to MEK7
  • Tested applicationsSuitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of human MEK7.

  • Positive control
    • HeLa cell lysate, human urinary bladder tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

     

    Alternative versions available:

    Anti-MEK7 antibody (Alexa Fluor® 647) [EP1455Y] (ab206232)

Properties

Applications

Our Abpromise guarantee covers the use of ab52618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 47 kDa.
IP 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • FunctionStress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
  • Tissue specificityUbiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by phosphorylation on Ser/Thr.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c-Jun N-terminal kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 7 antibody
    • Dual specificity mitogen-activated protein kinase kinase 7 antibody
    • JNK activating kinase 2 antibody
    • JNK kinase 2 antibody
    • JNK-activating kinase 2 antibody
    • JNKK 2 antibody
    • Jnkk-2 antibody
    • Jnkk2 antibody
    • MAP kinase kinase 7 antibody
    • MAP2K7 antibody
    • MAPK/ERK kinase 7 antibody
    • MAPKK 7 antibody
    • MAPKK-7 antibody
    • MAPKK7 antibody
    • MEK 7 antibody
    • Mitogen Activated Protein Kinase kinase 7 antibody
    • MKK 7 antibody
    • MKK-7 antibody
    • MKK7 antibody
    • MP2K7_HUMAN antibody
    • PRKMK 7 antibody
    • PRKMK-7 antibody
    • PRKMK7 antibody
    • SAPK kinase 4 antibody
    • SAPKK-4 antibody
    • SAPKK4 antibody
    • Sek 2 antibody
    • Sek-2 antibody
    • Sek2 antibody
    • SKK4 antibody
    • stress-activated protein kinase kinase 4 antibody
    see all

Anti-MEK7 antibody [EP1455Y] images



  • Predicted band size : 47 kDa

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: MEK7 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target - ab52618 observed at 47 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal
    ab52618 was shown to specifically react with MEK7 when MEK7 knockout samples were used. Wild-type and MEK7 knockout samples were subjected to SDS-PAGE. ab52618 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-MEK7 antibody [EP1455Y] (ab52618) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.

References for Anti-MEK7 antibody [EP1455Y] (ab52618)

This product has been referenced in:
  • Huang Y  et al. MAVS-MKK7-JNK2 defines a novel apoptotic signaling pathway during viral infection. PLoS Pathog 10:e1004020 (2014). Mouse . Read more (PubMed: 24651600) »
  • Lee CS  et al. MEK2 is sufficient but not necessary for proliferation and anchorage-independent growth of SK-MEL-28 melanoma cells. PLoS One 6:e17165 (2011). WB . Read more (PubMed: 21365009) »

See all 3 Publications for this product

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