Overview

  • Product name
    Anti-MEK7 antibody [EP1455Y]
    See all MEK7 primary antibodies
  • Description
    Rabbit monoclonal [EP1455Y] to MEK7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of human MEK7.

  • Positive control
    • HeLa cell lysate, human urinary bladder tissue
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 47 kDa.
IP 1/100.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
  • Tissue specificity
    Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by phosphorylation on Ser/Thr.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c-Jun N-terminal kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 7 antibody
    • Dual specificity mitogen-activated protein kinase kinase 7 antibody
    • JNK activating kinase 2 antibody
    • JNK kinase 2 antibody
    • JNK-activating kinase 2 antibody
    • JNKK 2 antibody
    • Jnkk-2 antibody
    • Jnkk2 antibody
    • MAP kinase kinase 7 antibody
    • MAP2K7 antibody
    • MAPK/ERK kinase 7 antibody
    • MAPKK 7 antibody
    • MAPKK-7 antibody
    • MAPKK7 antibody
    • MEK 7 antibody
    • Mitogen Activated Protein Kinase kinase 7 antibody
    • MKK 7 antibody
    • MKK-7 antibody
    • MKK7 antibody
    • MP2K7_HUMAN antibody
    • PRKMK 7 antibody
    • PRKMK-7 antibody
    • PRKMK7 antibody
    • SAPK kinase 4 antibody
    • SAPKK-4 antibody
    • SAPKK4 antibody
    • Sek 2 antibody
    • Sek-2 antibody
    • Sek2 antibody
    • SKK4 antibody
    • stress-activated protein kinase kinase 4 antibody
    see all

Images

  • Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: MEK7 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target - ab52618 observed at 47 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal
    ab52618 was shown to specifically react with MEK7 when MEK7 knockout samples were used. Wild-type and MEK7 knockout samples were subjected to SDS-PAGE. ab52618 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Anti-MEK7 antibody [EP1455Y] (ab52618) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.

References

This product has been referenced in:
  • Sheng X  et al. Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. PLoS Pathog 13:e1006534 (2017). Read more (PubMed: 28753655) »
  • Lukey MJ  et al. The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nat Commun 7:11321 (2016). Read more (PubMed: 27089238) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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