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Rabbit polyclonal to MEK7 (phospho S277 + T275)
Synthetic peptide conjugated to KLH, corresponding to amino acids surrounding Thr275/Ser277 of Human MEK7
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration. PubMed: 23226105
||1/1000. Predicted molecular weight: 47 kDa.
Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Activated by phosphorylation on Ser/Thr.
Information by UniProt
- c-Jun N-terminal kinase kinase 2 antibody
- Dual specificity mitogen activated protein kinase kinase 7 antibody
- Dual specificity mitogen-activated protein kinase kinase 7 antibody
Western blot - MEK7 (phospho S277 + T275) antibody (ab78148)
All lanes :
Anti-MEK7 (phospho S277 + T275) antibody (ab78148) at 1/1000 dilutionLane 1 :
Recombinant tagged MEK7ß1, untreatedLane 2 :
Recombinant tagged MEK7ß1, phosphorylated by MEKK1SecondaryAll lanes :
Goat anti-Rabbit HRP-conjugated at 1/5000 dilutionPredicted band size:
47 kDaObserved band size:
73 kDa (why is the actual band size different from the predicted?
Size of the protein tag alone: 26 kDa
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