Overview

  • Product nameAnti-MEK7 (phospho S277 + T275) antibody
    See all MEK7 primary antibodies
  • Description
    Rabbit polyclonal to MEK7 (phospho S277 + T275)
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids surrounding Thr275/Ser277 of Human MEK7

Properties

Applications

Our Abpromise guarantee covers the use of ab78148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23226105
WB 1/1000. Predicted molecular weight: 47 kDa.

Target

  • FunctionStress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
  • Tissue specificityUbiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by phosphorylation on Ser/Thr.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c-Jun N-terminal kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 7 antibody
    • Dual specificity mitogen-activated protein kinase kinase 7 antibody
    • JNK activating kinase 2 antibody
    • JNK kinase 2 antibody
    • JNK-activating kinase 2 antibody
    • Jnkk 2 antibody
    • Jnkk-2 antibody
    • Jnkk2 antibody
    • MAP kinase kinase 7 antibody
    • MAP2K7 antibody
    • MAPK/ERK kinase 7 antibody
    • MAPKK 7 antibody
    • MAPKK-7 antibody
    • MAPKK7 antibody
    • MEK 7 antibody
    • Mitogen Activated Protein Kinase kinase 7 antibody
    • MKK 7 antibody
    • MKK-7 antibody
    • MKK7 antibody
    • MP2K7_HUMAN antibody
    • PRKMK 7 antibody
    • PRKMK-7 antibody
    • PRKMK7 antibody
    • SAPK kinase 4 antibody
    • SAPKK-4 antibody
    • SAPKK4 antibody
    • Sek 2 antibody
    • Sek-2 antibody
    • Sek2 antibody
    • SKK4 antibody
    • stress-activated protein kinase kinase 4 antibody
    see all

Anti-MEK7 (phospho S277 + T275) antibody images

  • All lanes : Anti-MEK7 (phospho S277 + T275) antibody (ab78148) at 1/1000 dilution

    Lane 1 : Recombinant tagged MEK7ß1, untreated
    Lane 2 : Recombinant tagged MEK7ß1, phosphorylated by MEKK1

    Secondary
    Goat anti-Rabbit HRP-conjugated at 1/5000 dilution

    Predicted band size : 47 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)

References for Anti-MEK7 (phospho S277 + T275) antibody (ab78148)

This product has been referenced in:
  • Feltrin D  et al. Growth cone MKK7 mRNA targeting regulates MAP1b-dependent microtubule bundling to control neurite elongation. PLoS Biol 10:e1001439 (2012). WB, ICC/IF ; Mouse . Read more (PubMed: 23226105) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Mouse Tissue sections (Sciatic nerve)
Specification Sciatic nerve
Permeabilization Yes - 20 minutes at 98¯C in 10mM Tris Base, 1mM EDTA Solution, 0.05% Tween-20 in ddH2O, pH 9
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"