The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionComponent of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 RING-type zinc finger. Contains 1 SWIM-type zinc finger.
Mitogen activated protein kinase kinase kinase 1 antibody
Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody
Mitogen-activated protein kinase kinase kinase 1 antibody
Anti-MEKK1 antibody images
Western blot - MEKK1 antibody (ab55653)
Western blot against tagged recombinant protein immunogen using ab55653 MEKK1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MEKK1 antibody (ab55653)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
ab55653 staining MEKK1 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab55653 at a 1/200 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L) used at a 1/1000 dilution.