Overview

  • Product nameAnti-MEKK3 antibody [ep599y]
    See all MEKK3 primary antibodies
  • Description
    Rabbit monoclonal [ep599y] to MEKK3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N-terminal (Human)

  • Positive control
    • Hela cells; Human breast adenocarcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab40750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

Target

  • FunctionComponent of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

Anti-MEKK3 antibody [ep599y] images

  • Overlay histogram showing HeLa cells stained with ab40750 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40750, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-MEKK3 antibody [ep599y] (ab40750) at 1/1000 dilution + Hela cell lysate at 10 µg

    Predicted band size : 71 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of Hela cell lysate using ab40750.
  • Immunohistochemical analysis of paraffin embedded human breast adenocarcinoma using ab40750 diluted 1:50.
  • Immunofluorescent staining of HeLa cells using ab40750 diluted 1:50.

References for Anti-MEKK3 antibody [ep599y] (ab40750)

This product has been referenced in:
  • Chaveroux C  et al. Identification of a novel amino acid response pathway triggering ATF2 phosphorylation in mammals. Mol Cell Biol 29:6515-26 (2009). Read more (PubMed: 19822663) »
  • Matitau AE & Scheid MP Phosphorylation of MEKK3 at threonine 294 promotes 14-3-3 association to inhibit nuclear factor kappaB activation. J Biol Chem 283:13261-8 (2008). Read more (PubMed: 18308725) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"