Overview

  • Product name
    Anti-MEKK3 antibody [ep600y]
    See all MEKK3 primary antibodies
  • Description
    Rabbit monoclonal [ep600y] to MEKK3
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C-terminal (Human)

  • Positive control
    • HeLa cells, ovary carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab40756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

Target

  • Function
    Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

Images

  • All lanes : Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution

    Lane 1 : Recombinant human MEKK2 protein (ab135009)
    Lane 2 : Recombinant human MEKK3 protein (ab85297)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 71 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunofluorescent staining of HeLa cells using ab40756 diluted 1:100
  • Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Predicted band size : 71 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100

References

This product has been referenced in:

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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