Overview

  • Product nameAnti-Mel18 antibody - ChIP Grade
    See all Mel18 primary antibodies
  • Description
    Goat polyclonal to Mel18 - ChIP Grade
  • Tested applicationsSuitable for: IP, ICC/IF, ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: KMTVNGAPVPPLT, corresponding to C terminal amino acids 332-344 of Human Mel18.

  • Positive control
    • Human lung lysate.
  • General notesGene Ontology terms - transcription factor activity; regulation of transcription, DNA-dependent; nucleus. GenBank Accession Number – NP_009075.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. PubMed: 19636380
ICC/IF 1/100.
ChIP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with Human Mel18 peptide (ab23085).

Target

  • FunctionTranscriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Tissue specificityDetected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein Mel 18 antibody
    • DNA-binding protein Mel-18 antibody
    • Mel 18 antibody
    • MGC10545 antibody
    • PCGF 2 antibody
    • PCGF2 antibody
    • PCGF2_HUMAN antibody
    • Polycomb group ring finger 2 antibody
    • Polycomb group RING finger protein 2 antibody
    • RING finger protein 110 antibody
    • RNF110 antibody
    • Zinc finger protein 144 antibody
    • ZNF 144 antibody
    • ZNF144 antibody
    see all

Anti-Mel18 antibody - ChIP Grade images



  • Predicted band size : 40 kDa


    ab5267 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References for Anti-Mel18 antibody - ChIP Grade (ab5267)

This product has been referenced in:
  • Klauke K  et al. Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation. Nat Cell Biol 15:353-62 (2013). WB . Read more (PubMed: 23502315) »
  • Holdt LM  et al. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet 9:e1003588 (2013). Read more (PubMed: 23861667) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (NT2 cell line)
Negative control Intergenic region on Chromosome 11.
Specification NT2 cell line
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control CCND2 and ALX4 genes
Username

tatiana kan

Verified customer

Submitted Oct 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (NB4 cell line(acute promylocytic leukemia))
Specification NB4 cell line(acute promylocytic leukemia)
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. sungsin jo

Verified customer

Submitted Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (HL60(acute promyelotic leuemia cells line))
Specification HL60(acute promyelotic leuemia cells line)
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: SDS lysis buffer
Detection step Semiquantitative PCR
Positive control acetly-histone H3 (cat #06-599, millipore)
Negative control Rabbit IgG(cat# pp64, millipore)
Username

Mr. sungsin jo

Verified customer

Submitted Oct 23 2009

Thank you for your enquiry. I have consulted the EXPASY databases and I retrieved the following information: Mel18 - Tissue specificity: Detected in all tissues examined with high expression found in placenta, lung and kidney and low expression, in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"