Anti-Mel18 antibody (ab5267)
Key features and details
- Goat polyclonal to Mel18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Mel18 antibody -
Description
Goat polyclonal to Mel18 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Mel18 aa 300-400 (C terminal).
Database link: NP_009075.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). A 1 hour primary incubation is recommended for this product. |
Target
-
Function
Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
Tissue specificity
Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsPhosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7703 Human
- Omim: 600346 Human
- SwissProt: P35227 Human
- Unigene: 371617 Human
- Unigene: 713595 Human
-
Alternative names
- DNA binding protein Mel 18 antibody
- DNA-binding protein Mel-18 antibody
- Mel 18 antibody
see all
Images
-
ab5267 staining (1
ab5267 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.µ g/ml) of Human Lung lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
-
Datasheet download
References (15)
ab5267 has been referenced in 15 publications.
- Gonzalez MM et al. BMI1 maintains the Treg epigenomic landscape to prevent inflammatory bowel disease. J Clin Invest 131:N/A (2021). PubMed: 34128475
- Paschos K et al. Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C. Nucleic Acids Res N/A:N/A (2019). PubMed: 30649516
- Wang J et al. Co-inhibition of BMI1 and Mel18 enhances chemosensitivity of esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 17:5012-5022 (2019). PubMed: 31186712
- Klauke K et al. Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation. Nat Cell Biol 15:353-62 (2013). WB . PubMed: 23502315
- Holdt LM et al. Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks. PLoS Genet 9:e1003588 (2013). PubMed: 23861667