For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.|
|IHC-Fr||1/50 - 1/100. ABC method.
It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas.
Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.