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Other Immunogen Type corresponding to Melanoma. Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.
Isotypes: IgG1/kappa, IgG1 and IgG2a.
The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.
Our Abpromise guarantee covers the use of ab82762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|