Anti-Melanoma antibody [Cocktail - HMB45+A103+T311], prediluted (ab82762)

Overview

  • Product name
    Anti-Melanoma antibody [Cocktail - HMB45+A103+T311], prediluted
    See all Melanoma primary antibodies
  • Description
    Mouse monoclonal [Cocktail - HMB45+A103+T311] to Melanoma, prediluted
  • Specificity
    This antibody has been shown to label the majority of melanomas.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Melanoma. Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.

  • Positive control
    • Metastatic melanoma in lymph node.
  • General notes

    Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, Stabilizing agent, pH 7.5
  • Concentration information loading...
  • Primary antibody notes
    Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.
  • Clonality
    Monoclonal
  • Clone number
    Cocktail - HMB45+A103+T311
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/1.

Target

  • Relevance
    Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Cellular localization
    Membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Antigen LB39 AA antibody
    • Antigen SK29 AA antibody
    • MART1 antibody
    • melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • Monophenol monooxygenase antibody
    • Tumor rejection antigen AB antibody
    • tyrosinase antibody
    see all

References

This product has been referenced in:
  • Kapur RP  et al. Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes. J Histochem Cytochem 40:207-12 (1992). IHC-P, IHC-Fr . Read more (PubMed: 1552165) »
  • Gown AM  et al. Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol 123:195-203 (1986). IHC-P, IHC-Fr . Read more (PubMed: 3518473) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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