Recombinant Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311] (ab733)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse recombinant multiclonal [HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma
- Suitable for: ICC/IF, Flow Cyt (Intra), IHC-Fr, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3 + T311]
See all Melanoma primary antibodies -
Description
Mouse recombinant multiclonal [HMB45 + M2-7C10 + M2-9E3 + T311] to Melanoma -
Host species
Mouse -
Specificity
The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. MART-1 recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein Tyrosinase - Recombinant tyrosinase protein
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Positive control
- Metastatic melanoma in lymph node.
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General notes
The combination of HMB45, MART-1 (M2-7C10 + M2-9E3) and Tyrosinase (T311) (SwissProt: P14679 Human, Omim: 606933 Human, Entrez Gene: 22173 Mouse, Entrez Gene: 308800 Rat) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, Carrier protein -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Antibodies in this cocktail- HMB45, MART-1 and Tyrosinase are affinity purified. -
Primary antibody notes
The combination of HMB45, MART-1 (DT101 + BC199) and Tyrosinase (T311) make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes (see reference 3.). -
Clonality
Recombinant Multiclonal -
Clone number
HMB45 + M2-7C10 + M2-9E3 + T311 -
Myeloma
unknown -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
1/100.
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IHC-Fr |
Use at an assay dependent concentration.
ABC method. |
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IHC-P | (1) |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
1/100. |
IHC-Fr
Use at an assay dependent concentration. ABC method. |
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol. |
Target
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Relevance
Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Cellular localization
Membrane; Single-pass membrane protein -
Database links
- Omim: 605513 Human
- SwissProt: Q16655 Human
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Alternative names
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
Images
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IHC image of ab733 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab733, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ab733 staining human melanoma by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Overlay histogram showing MALME 3M cells stained with ab733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab733, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab733 has been referenced in 11 publications.
- Shi C et al. Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line. Cancer Commun (Lond) 42:627-647 (2022). PubMed: 35666052
- Ou L et al. Preclinical platforms to study therapeutic efficacy of human γδ T cells. Clin Transl Med 12:e814 (2022). PubMed: 35731974
- Su CY et al. Successful Repigmentation of Full-Thickness Wound Healing in Fraser's Dolphins (Lagenodelphis hosei). Animals (Basel) 12:N/A (2022). PubMed: 35739819
- Shao H et al. Converting melanoma-associated fibroblasts into a tumor-suppressive phenotype by increasing intracellular Notch1 pathway activity. PLoS One 16:e0248260 (2021). PubMed: 33705467
- Ikeda Y et al. Melanocyte progenitor cells reside in human subcutaneous adipose tissue. PLoS One 16:e0256622 (2021). PubMed: 34432824