Overview

  • Product nameAnti-Melanoma antibody [HMB45]
    See all Melanoma primary antibodies
  • Description
    Mouse monoclonal [HMB45] to Melanoma
  • SpecificityThis antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.
  • Tested applicationsFlow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Extract of pigmented melanoma metastases from lymph nodes.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Tissue culture supernatant
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberHMB45
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.
IHC-P 1/10 - 1/50. ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Enzymatic antigen retrieval may increase staining intensity.
IHC-Fr 1/10 - 1/50. ABC method.
ICC/IF Use at an assay dependent dilution. PubMed: 19841138

Target

  • RelevanceMalignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Cellular localizationMembrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Antigen LB39 AA antibody
    • Antigen SK29 AA antibody
    • CMM 1 antibody
    • CMM antibody
    • CMM1 antibody
    • Cutaneous Malignant Melanoma Dysplastic Nevus antibody
    • DNS antibody
    • Dysplastic Nevus Syndrome antibody
    • FAMMM antibody
    • MART1 antibody
    • melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • MLM antibody
    • Monophenol monooxygenase antibody
    • Tumor rejection antigen AB antibody
    • tyrosinase antibody
    see all

Anti-Melanoma antibody [HMB45] images

  • ab787 - immunohistochemistry

    Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

  • ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

    See Abreview

  • Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Melanoma antibody [HMB45] (ab787)

This product has been referenced in:
  • Lazova R  et al. Punctate LC3B expression is a common feature of solid tumors and associated with proliferation, metastasis, and poor outcome. Clin Cancer Res 18:370-9 (2012). Human . Read more (PubMed: 22080440) »
  • Kästle M  et al. Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes. Free Radic Biol Med 50:305-12 (2011). ICC/IF, Flow Cyt ; Human . Read more (PubMed: 21078385) »

See all 4 Publications for this product

Product Wall



We don't have strict guidelines for length of storage for antibodies. In general, for antibody products stored at 4 degrees C, we would expect them to be stable for at least one year from date of purchase.

Merci de nous avoir contactés; ma collègue m'a fait part de votre appel.

Je suis heureux de savoir que l'anticorps a finalement fonctionné en cytométrie en flux. J'ai compris que vous deviez utiliser beaucoup d'anti...

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Thank you for contacting us.

Unfortunately, the blocking peptide for ab787 antibody is not available for purchase. We have ˜25 anti melanoma antibodies available but we do not have antigen available for any one of these however if you could se...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma cell line WM3248 xenopraph)
Specification Melanoma cell line WM3248 xenopraph
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Permeabilization Yes - 0.1% triton x-100
Blocking step Dakocytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Hongwei Shao

Verified customer

Submitted Feb 25 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (transfected HeLa)
Loading amount 20 µg
Specification transfected HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Guillaume van Niel

Verified customer

Submitted Jul 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"