Anti-Melanoma Associated Antigen KBA.62 antibody [KBA.62] (ab101138)

Overview

  • Product name
    Anti-Melanoma Associated Antigen KBA.62 antibody [KBA.62]
  • Description
    Mouse monoclonal [KBA.62] to Melanoma Associated Antigen KBA.62
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Melanoma Associated Antigen

  • Positive control
    • Human Melanoma
  • General notes


    ab101138 is a novel anti-melanoma antibody. Studies thus far have shown a similar sensitivity to melanocytic proliferations as that seen with S100 protein staining, which is somewhat higher than that seen with HMB45. This has been confirmed by one study on a series of 215 sentinel lymph nodes. Moreover, ab101138 identified 6 patients (3%) who had confirmed sentinel lymph node metastasis but stained negative for HMB45. In this setting, the resolution appears to be higher than S100 protein in that the staining pattern (membranous) is quite distinct. Interestingly, most cases of desmoplastic and spindle cell melanomas show strongly positive results with ab101138, unlike that seen with other melanocyte markers. It should be noted that ab101138 will label occasional endothelial cells which can serve as an internal positive control. A small percentage of well differentiated squamous cell carcinomas of the skin (and lung) have also been noted to stain with this antibody; however, the poorly differentiated forms of carcinoma do not, thus resolving a greater practical problem in differential diagnosis. Therefore, ab101138 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micrometastasis in sentinel lymph node.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, BSA, pH 7.4
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    ab101138 is a novel anti-melanoma antibody. Studies thus far have shown a similar sensitivity to melanocytic proliferations as that seen with S100 protein staining, which is somewhat higher than that seen with HMB45. This has been confirmed by one study on a series of 215 sentinel lymph nodes. Moreover, ab101138 identified 6 patients (3%) who had confirmed sentinel lymph node metastasis but stained negative for HMB45. In this setting, the resolution appears to be higher than S100 protein in that the staining pattern (membranous) is quite distinct. Interestingly, most cases of desmoplastic and spindle cell melanomas show strongly positive results with ab101138, unlike that seen with other melanocyte markers. It should be noted that ab101138 will label occasional endothelial cells which can serve as an internal positive control. A small percentage of well differentiated squamous cell carcinomas of the skin (and lung) have also been noted to stain with this antibody; however, the poorly differentiated forms of carcinoma do not, thus resolving a greater practical problem in differential diagnosis. Therefore, ab101138 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micrometastasis in sentinel lymph node.
  • Clonality
    Monoclonal
  • Clone number
    KBA.62
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
IHC-Fr 1/25 - 1/100.

References

ab101138 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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