• Product nameAnti-Melanoma gp100 antibody [7E3]
    See all Melanoma gp100 primary antibodies
  • Description
    Mouse monoclonal [7E3] to Melanoma gp100
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Melanoma gp100. Full length recombinant Human Melanoma gp100 (NP_008859) produced in HEK293T cells.
    Database link: P40967

  • Positive control
    • HEK293T transfected cells



Our Abpromise guarantee covers the use of ab117853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 23389629


  • FunctionPlays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
  • Tissue specificityPreferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
  • Sequence similaritiesBelongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • DomainThe RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
    The lumenal domain is necessary for correct processing and trafficking to melanosomes.
  • Post-translational
    A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
    N-glycosylated. O-glycosylated; contains sialic acid.
  • Cellular localizationSecreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 kDa melanocyte specific secreted glycoprotein antibody
    • 95 kDa melanocyte-specific secreted glycoprotein antibody
    • D12S53E antibody
    • gp100 antibody
    • M-beta antibody
    • ME20 antibody
    • ME20 M/ME20 S antibody
    • ME20-M antibody
    • ME20-S antibody
    • ME20M antibody
    • ME20M/ME20S antibody
    • ME20S antibody
    • Melanocyte lineage specific antigen GP100 antibody
    • Melanocyte protein mel 17 antibody
    • Melanocyte protein Pmel 17 antibody
    • Melanocyte protein Pmel 17 precursor antibody
    • Melanocytes lineage-specific antigen GP100 antibody
    • Melanoma associated ME20 antigen antibody
    • Melanoma gp100 antibody
    • Melanoma-associated ME20 antigen antibody
    • Melanosomal matrix protein 17 antibody
    • Melanosomal matrix protein17 antibody
    • P1 antibody
    • p100 antibody
    • p26 antibody
    • PMEL 17 antibody
    • PMEL antibody
    • PMEL_HUMAN antibody
    • PMEL17 antibody
    • Premelanosome protein antibody
    • Secreted melanoma-associated ME20 antigen antibody
    • SI antibody
    • SIL antibody
    • SILV antibody
    • Silver (mouse homolog) like antibody
    • Silver homolog antibody
    • Silver locus protein homolog antibody
    • Silver, mouse, homolog of antibody
    see all

Anti-Melanoma gp100 antibody [7E3] images

  • Flow cytometry image using ab117853 at a 1/100 dilution, staining HEK293T cells transfected with Melanoma gp100 (Red), or an empty vector control plasmid (Blue).

References for Anti-Melanoma gp100 antibody [7E3] (ab117853)

This product has been referenced in:
  • Leonhardt RM  et al. Critical residues in the PMEL/Pmel17 N-terminus direct the hierarchical assembly of melanosomal fibrils. Mol Biol Cell : (2013). ICC/IF, Flow Cyt ; Human . Read more (PubMed: 23389629) »

See 1 Publication for this product

Product Wall

Following up my message from Thursday, I was just informed that the originating laboratory for gp100 antibody ab117853 did test western blotting using overexpression lysates and it failed to detect the overexpressed protein. As I mentioned, a blot of n...

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After some discussion with our source for the Anti-Melanoma gp100 antibody, clone7E3, catalogue ID ab117853, I have learned that it has apparently either never been tested for western blotting before, or was tested and failed. I am still waiting for cl...

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Thank you for contacting us.

Regarding your second question about anti-Pmel17 ab117853, clone 7E3, I am waiting for the lab to send a western blot protocol. I expect it will be standard. Can you tell me how much sample you are loading per la...

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Thank you for your inquiry and your interest in our antibodies. The recommendation you found on the website, was specific in regards to make a double staining with another specific antibody.

I can recommend you to follow the classical intrace...

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Thank you fagain for your interest in our products.

I have attached the protocol information provide to me by the lab for your review.

Again, I am sorry but we are not aware of any specific references to this product in publicat...

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Thank you for your response.

This product is tested on permeablized cells. I have attached the QA protocol for this product for your review as well.

As this is a relatively new product, we are not currently aware of any publicatio...

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Thank you for contacting us.

The secondary antibody which had been used with the flow cytometry data presented on our datasheet is Goat anti-Mouse Alexa 488.

This particular product is a relatively new addition to Abcam as we are ...

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Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have onl...

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