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CPIGENSPLLSGQQ, corresponding to C terminal amino acids 647-660 of Human Melanoma gp100
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Primary incubation was 1 hour. Detected by chemiluminescence. The 26kDa band observed in Human Skin lysate corresponds to the precusor protein M-beta fragment (Leonhardt et al, Mol Biol Cell. 2013 Apr;24(7):964-81.PMID: 23389629).
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