Anti-Melanoma Inhibitory Activity antibody (ab110612)

Overview

  • Product nameAnti-Melanoma Inhibitory Activity antibody
    See all Melanoma Inhibitory Activity primary antibodies
  • Description
    Rabbit polyclonal to Melanoma Inhibitory Activity
  • Tested applicationsSuitable for: Flow Cyt, Indirect ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Highly pure (>98%) recombinant Human Melanoma Inhibitory Activity

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Melanoma Inhibitory Activity.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin) (ab84235). Using ab84235 as the detection antibody and ab110612 as the capture antibody, the detection limit for recombinant Human Melanoma Inhibitory Activity is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Human Melanoma Inhibitory Activity is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • Sequence similaritiesBelongs to the MIA/OTOR family.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    May possess two intramolecular disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage derived retinoic acid sensitive protein antibody
    • CD RAP antibody
    • CDRAP antibody
    • Melanoma derived growth regulatory protein precursor antibody
    • Melanoma inhibitory activity protein antibody
    • Melanoma-derived growth regulatory protein antibody
    • Mia antibody
    • MIA_HUMAN antibody
    see all

Anti-Melanoma Inhibitory Activity antibody images

  • ab110612 staining Melanoma Inhibitory Activity in Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/1000 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

     

    See Abreview

References for Anti-Melanoma Inhibitory Activity antibody (ab110612)

ab110612 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - Saponine
Sample Human Cell (melanoma cell line)
Specification melanoma cell line
Gating Strategy cell line only
Preparation Cell harvesting/tissue preparation method: EDTA (Versen) for harvesting cell
Sample buffer: PBS
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Submitted Jul 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"