Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Human Melanoma Inhibitory Activity is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionElicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Tissue specificityAll malignant melanoma cell lines tested and infrequently in glioma cell lines.
Sequence similaritiesBelongs to the MIA/OTOR family. Contains 1 SH3 domain.
Post-translational modificationsMay possess two intramolecular disulfide bonds.
Cartilage derived retinoic acid sensitive protein antibody
CD RAP antibody
Melanoma derived growth regulatory protein precursor antibody
Melanoma inhibitory activity protein antibody
Melanoma-derived growth regulatory protein antibody
Anti-Melanoma Inhibitory Activity antibody images
Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (ab110612)This image is courtesy of an anonymous Abreview
ab110612 staining Melanoma Inhibitory Activity in Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/1000 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.