Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)

Overview

  • Product name
    Anti-Melanoma Inhibitory Activity antibody (Biotin)
    See all Melanoma Inhibitory Activity primary antibodies
  • Description
    Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant full length Human Melanoma Inhibitory Activity

Properties

Applications

Our Abpromise guarantee covers the use of ab84235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
Flow Cyt
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 15 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
    • Tissue specificity
      All malignant melanoma cell lines tested and infrequently in glioma cell lines.
    • Sequence similarities
      Belongs to the MIA/OTOR family.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      May possess two intramolecular disulfide bonds.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cartilage derived retinoic acid sensitive protein antibody
      • CD RAP antibody
      • CDRAP antibody
      • Melanoma derived growth regulatory protein precursor antibody
      • Melanoma inhibitory activity protein antibody
      • Melanoma-derived growth regulatory protein antibody
      • Mia antibody
      • MIA_HUMAN antibody
      see all

    Images

    • ab84235 staining Melanoma Inhibitory Activity in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    References

    ab84235 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - Saponine
    Sample
    Human Cell (melanoma cell line)
    Specification
    melanoma cell line
    Gating Strategy
    cell line only
    Preparation
    Cell harvesting/tissue preparation method: EDTA (Versen) for harvesting cell
    Sample buffer: PBS
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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