Anti-Melanophilin antibody (ab2716)

Overview

  • Product nameAnti-Melanophilin antibody
    See all Melanophilin primary antibodies
  • Description
    Goat polyclonal to Melanophilin
  • Specificityab2716 recognises Melanophilin.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    ARHIFAKPVMAQQP

    , corresponding to C terminal amino acids 577-590 of Mouse Melanophilin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionRab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • Involvement in diseaseDefects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2210418F23Rik antibody
    • 5031433I09Rik antibody
    • AW228792 antibody
    • D1Wsu84e antibody
    • Exophilin 3 antibody
    • Exophilin-3 antibody
    • l(1)-3Rk antibody
    • l1Rk3 antibody
    • Leaden antibody
    • Leaden protein antibody
    • ln antibody
    • Melanophilin antibody
    • MELPH_HUMAN antibody
    • MGC2771 antibody
    • MGC59733 antibody
    • Mlph antibody
    • Slac 2a antibody
    • SlaC2-a antibody
    • Slp homolog lacking C2 domains a antibody
    • Synaptotagmin like protein 2a antibody
    • Synaptotagmin like protein lacking C2 domains A antibody
    • Synaptotagmin-like protein 2a antibody
    see all

Anti-Melanophilin antibody images

  • All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml

    Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
    Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice


    Predicted band size : 65 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    ab2716 staining (3µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence.
  • Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

References for Anti-Melanophilin antibody (ab2716)

ab2716 has not yet been referenced specifically in any publications.

Product Wall

This antibody has not yet been tested for use in IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"