Overview

  • Product name
  • Description
    Rabbit polyclonal to Melanopsin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KMNSPSESRVPSSLTQDPSF

    , corresponding to amino acids 1-19 of Rat Melanopsin.

  • Positive control
    • ICC: rat retinal ganglion cells WB: overexpressed melanopsin in HEK293 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.
  • Tissue specificity
    Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • Cellular localization
    Cell membrane. Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Melanopsin antibody
    • MGC142118 antibody
    • MOP antibody
    • OPN 4 antibody
    • OPN4 antibody
    • OPN4_HUMAN antibody
    • Opsin 4 antibody
    • Opsin-4 antibody
    • Opsin4 antibody
    see all

Images

  • Immunofluorescence and immunocytochemistry staining of Melanopsin in rat retinal ganglion cells (RGCs) can be performed using ab19306.

  • By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated rat melanopsin from HEK 293 cells.

References

This product has been referenced in:
  • Boudard DL  et al. Loss of photic entrainment at low illuminances in rats with acute photoreceptor degeneration. Eur J Neurosci 30:1527-36 (2009). Read more (PubMed: 19821841) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your reply. I am glad you found the information I sent helpful. I hope you obtain the staining you are hoping for with the Melanopsin antibody you have purchased. If I can be of any further assistance, please do not hesitate to let me kno...

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Thank you for contacting us yesterday in regards to the Melanopsin antibodies available from our catalogue. I am sorry for the delay in my reply.

As discussed over the phone, I have checked if we have any images available for the results obtai...

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Thank you for your enquiry. I am sorry to hear you are having difficulty obtaining satisfactory results from this antibody (ab19306). This antibody has been tested on total protein extracts of HEK293 cells with or without a c-myc tag (to verify the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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