The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionHigh affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
Tissue specificityExpressed in hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN). Hippocampus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Western blot - Anti-Melatonin Receptor 1A antibody (ab116337)
Anti-Melatonin Receptor 1A antibody (ab116337) at 1 µg/ml + Jurkat cell lysate at 10 µg
Predicted band size : 39 kDa
References for Anti-Melatonin Receptor 1A antibody (ab116337)
This product has been referenced in:
Guo P et al. Melatonin Improves Mitochondrial Function by Promoting MT1/SIRT1/PGC-1 Alpha-Dependent Mitochondrial Biogenesis in Cadmium-Induced Hepatotoxicity In Vitro. Toxicol SciN/A:N/A (2014).
Read more (PubMed: 25159133) »