Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab87639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
  • Tissue specificity
    Expressed in hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN). Hippocampus.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mel 1A R antibody
    • MEL-1A-R antibody
    • Mel1a melatonin receptor antibody
    • Mel1a receptor antibody
    • Melatonin receptor 1A antibody
    • Melatonin receptor type 1A antibody
    • MelR antibody
    • MR antibody
    • MT 1 antibody
    • MT1 antibody
    • MTNR 1A antibody
    • MTNR1A antibody
    • MTR1A_HUMAN antibody
    see all

Images

  • Anti-Melatonin Receptor 1A antibody (ab87639) at 0.3 µg/ml + KELLY lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References

This product has been referenced in:
  • Villarreal MA  et al. Determining Zebrafish Epitope Reactivity to Commercially Available Antibodies. Zebrafish 14:387-389 (2017). Read more (PubMed: 28318435) »
  • Mori F  et al. Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects. Oncotarget 7:20532-48 (2016). WB . Read more (PubMed: 26967561) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Hypothalamus, Pars distalis pituitary, Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Hypothalamus, Pars distalis pituitary, Retina
Blocking step
Hydrogen peroxide as blocking agent for 30 minute(s) · Concentration: 0.3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Tulika Gupta

Verified customer

Submitted Dec 22 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Sample
Zebrafish Tissue sections (Retina, Brain)
Specification
Retina, Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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