Overview

  • Product name
    Anti-MELK antibody [2G2]
    See all MELK primary antibodies
  • Description
    Mouse monoclonal [2G2] to MELK
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide:

    C-VYKRLVEDILSSCKV

    (with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680).

  • Positive control
    • HepG2 cells and MCF-7 cells. Human liver cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab129373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function
    Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
  • Tissue specificity
    Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 KA1 (kinase-associated) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI327312 antibody
    • hMELK antibody
    • HPK 38 antibody
    • hPK38 antibody
    • KIAA0175 antibody
    • Likely ortholog of maternal embryonic leucine zipper kinase antibody
    • Maternal embryonic leucine zipper kinase antibody
    • MELK antibody
    • MELK_HUMAN antibody
    • mKIAA0175 antibody
    • MPK38 antibody
    • OTTHUMP00000021377 antibody
    • OTTHUMP00000046113 antibody
    • pEg3 kinase antibody
    • Protein kinase Eg3 antibody
    • Protein kinase PK38 antibody
    • RP23 382O11.1 antibody
    • Tyrosine protein kinase MELK antibody
    see all

Images

  • ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
  • ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
  • ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
  • ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.

References

ab129373 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129373.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up