Overview

  • Product name
    Anti-MELK antibody [EPR3981]
    See all MELK primary antibodies
  • Description
    Rabbit monoclonal [EPR3981] to MELK
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MELK (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: K562, 293T, and Jurkat cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
    • Tissue specificity
      Expressed in placenta, kidney, thymus, testis, ovary and intestine.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI327312 antibody
      • hMELK antibody
      • HPK 38 antibody
      • hPK38 antibody
      • KIAA0175 antibody
      • Likely ortholog of maternal embryonic leucine zipper kinase antibody
      • Maternal embryonic leucine zipper kinase antibody
      • MELK antibody
      • MELK_HUMAN antibody
      • mKIAA0175 antibody
      • MPK38 antibody
      • OTTHUMP00000021377 antibody
      • OTTHUMP00000046113 antibody
      • pEg3 kinase antibody
      • Protein kinase Eg3 antibody
      • Protein kinase PK38 antibody
      • RP23 382O11.1 antibody
      • Tyrosine protein kinase MELK antibody
      see all

    Images



    • Predicted band size : 75 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MELK knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HCT116 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 75 kDa

    References

    This product has been referenced in:
    • Lin A  et al. CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials. Elife 6:N/A (2017). Read more (PubMed: 28337968) »
    • Wang Y  et al. Mitotic MELK-eIF4B signaling controls protein synthesis and tumor cell survival. Proc Natl Acad Sci U S A 113:9810-5 (2016). Read more (PubMed: 27528663) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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