Membrane Integrity WB Antibody Cocktail (ab110414)

Overview

  • Product nameMembrane Integrity WB Antibody Cocktail
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:

    Outer Membrane - Porin ab14734 (MSA03)

    Intermembrane Space - Cytochrome c ab110325 (MSA06)

    Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)

    Matrix Space - Cyclophilin D ab110324 (MSA04)

    This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D. Human heart mitochondria are available as standard control (ab110337) mitochondria from other species and tissues are available.

    Cocktail Antibodies:

    Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
    Amount: 50ug
    Working concentration: 2ug/ml

    Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
    Amount: 50ug
    Working concentration: 2ug/ml

    Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
    Amount: 50ug
    Working concentration: 2ug/ml

    Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
    Amount: 5ug
    Working concentration: 0.1ug/ml

    Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
    Amount: 100ug
    Working concentration: 4ug/ml

  • Notes

    Store cocktail at 4°C and control at -80°C.

  • Tested applicationsSuitable for: WBmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab110414 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.

    Membrane Integrity WB Antibody Cocktail images

    • All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)

      Lane 1 : Rat liver mitos prep #1
      Lane 2 : Rat liver mitos prep #2

      Lysates/proteins at 10 µg per lane.

    References for Membrane Integrity WB Antibody Cocktail (ab110414)

    This product has been referenced in:
    • Melis N  et al. Targeting eIF5A Hypusination Prevents Anoxic Cell Death through Mitochondrial Silencing and Improves Kidney Transplant Outcome. J Am Soc Nephrol N/A:N/A (2016). Read more (PubMed: 27612998) »
    • Baudot AD  et al. Using enhanced-mitophagy to measure autophagic flux. Methods 75:105-11 (2015). WB . Read more (PubMed: 25498004) »

    See all 6 Publications for this product

    Product Wall

    Abreviews
    Application: Western Blot
    Sample: Mouse whole cell lysate (3T3 SA cell line)
    Loading amount: 20ug
    Gel Running conditions: reduced denaturating (4-12% gradient gel)
    Blocking step: 30 min in 5% milk at room temperature
    Dilution: 1/1000
    Incubation time: over night at 4°C or 1 hour at room temperature
    Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
    Detection: ECL
    Username

    Abcam user community

    Verified customer

    Submitted Aug 21 2014

    Thank you for your enquiry.

    MitoSciences Sucrose gradient (ab110414) protocol is a protein subfractionation method optimized for mitochondria. Therefore, the starting material is enriched mitochondria.

    To fractionation mitochondrial...

    Read More

    Gracias por contactarnos.

    Atendiendo a la imagen enviada, las bandas parecen corresponder con las esperadas correspondientes a las distintas estructuras mitocondriales.

    Es muy posible que la banda más intensa que sale entre 5...

    Read More

    Thank for your reply.

    1. The MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414) should be suitable for whole cell extracts. You may observe some background staining as the product has been optimized on Mitochondrial extracts, an...

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    Vielen Dank für die Zusammenfassung in Englisch. Ich habe nun eine Antwort von unseren Mitochondrien -Spezialisten aus dem Labor erhalten.

    Ich habe die Antwort nicht übersetzt um Übersetzungsfehler zu vermeiden:

    "...

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    Thank you for your inquiry.


    Yes, you use a cocktail antibody in the same way as a regular antibody in WB. You'll need an anti-mouse IgGHRP conjugated antibody. You can load 10ug of the rat liver mitochondria as a control.

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    Thank you for your inquiry. I am happy to confirm the amounts of positive control per gel: Please use 10ug of the rat liver prep each lane or any positive control that is given. I hope this information is helpful and wish you a good start into the new ...

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    Thank you for your reply. I have been in touch with our colleagues at Mitosciences who have kindly provided the following information: To answer your question directly, The antibody coating the plate supplied with Kit MTOX1 ab109903 does not cr...

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    Thank you for contacting us with your questions about ab110414. The antibodies will be provided in one tube, at a total concentration of 2.37 mg/mL. The individual amounts of each antibody are available on the datasheet. We recommend using the cocktail...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"