The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
Use a concentration of 2 - 10 µg/ml.
FunctionEssential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
Involvement in diseaseDefects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia. Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
Detection of Human Menin by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate (40 mcg - E; 10 mcg - T) from 293T cells untreated (E) or transfected with a Menin expression construct (T). Antibodies: A. ab11894 used at the indicated concentrations for WB. B. Affinity purified rabbit anti-Menin antibodies ab2605 (5 mcg/plate), ab11893 (10 mcg/plate) and ab11894 (10 mcg/plate) for IP. Immunoprecipitated Menin was detected using ab2605 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times of 1 minute (A) or 1 second (B).
References for Anti-Menin antibody (ab11894)
has not yet been referenced specifically in any publications.
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