• Product nameAnti-Menin antibody [EPR3986]
    See all Menin primary antibodies
  • Description
    Rabbit monoclonal [EPR3986] to Menin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C terminus of Human Menin.

  • Positive control
    • Jurkat, 293T, K-562, A431 and HeLa cell lysates; Human colon tissue; Human papillary carcinoma of thyroid gland; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab92443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionEssential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseDefects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
      Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
    • Information by UniProt
    • Database links
    • Alternative names
      • MEA 1 antibody
      • MEA1 antibody
      • MEN 1 antibody
      • Men1 antibody
      • MEN1_HUMAN antibody
      • Menin antibody
      • Multiple Endocrine Adenomatosis 1 antibody
      • Multiple Endocrine Neoplasia 1 antibody
      • SCG 2 antibody
      • SCG2 antibody
      • Suppressor Candidate Gene 2 antibody
      • Wermer syndrome antibody
      • ZES antibody
      • Zollinger Ellison Syndrome antibody
      see all

    Anti-Menin antibody [EPR3986] images

    • Predicted band size : 68 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Menin knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92443 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab92443 was shown to recognize Menin when Menin knockout samples were used, along with additional cross-reactive bands. Wild-type and Menin knockout samples were subjected to SDS-PAGE. ab92443 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Menin antibody [EPR3986] (ab92443) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K-562 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 68 kDa
    • ab92443, at 1/100 dilution, staining Menin in paraffin-embedded (A) Human colon tissue and (B) Human papillary carcinoma of thyroid gland by Immunohistochemistry.
    • ab92443, at 1/250 dilution, staining Menin in HeLa cells by Immunofluorescence.
    • ab92443 showing positive staining in Breast carcinoma tissue.

    • ab92443 showing positive staining in Colonic adenocarcinoma tissue.

    • ab92443 showing positive staining in Lung adenocarcinoma tissue.

    • ab92443 showing positive staining in Glioma tissue.

    References for Anti-Menin antibody [EPR3986] (ab92443)

    This product has been referenced in:
    • Hamze Z  et al. Altered MENIN expression disrupts the MAFA differentiation pathway in insulinoma. Endocr Relat Cancer 20:833-48 (2013). IHC ; Human . Read more (PubMed: 24157940) »

    See 1 Publication for this product

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