Overview

  • Product nameAnti-MEPCE antibody
    See all MEPCE primary antibodies
  • Description
    Goat polyclonal to MEPCE
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    KQQRKFQYGNYCK

    , corresponding to internal sequence amino acids 408-420 of Human MEPCE (NP_062552.2).

  • Positive control
    • Rat testis lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77329 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevanceMEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.
  • Database links
  • Alternative names
    • 7SK snRNA methylphosphate capping enzyme antibody
    • BCDIN3 antibody
    • Bicoid interacting protein 3 homolog antibody
    • Bin3 bicoid interacting 3 antibody
    • Bin3 bicoid interacting 3 homolog (Drosophila) antibody
    • Bin3 bicoid interacting 3 homolog antibody
    • Bin3 homolog antibody
    • FLJ20257 antibody
    • MePCE antibody
    • Methylphosphate capping enzyme antibody
    see all

Anti-MEPCE antibody images

  • ab77329 (5µg/ml) staining of MEPCE in paraffin embedded Human Testis following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of the spermatids.

  • Anti-MEPCE antibody (ab77329) at 1 µg/ml + rat testis lysate in RIPA buffer at 35 µg

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-MEPCE antibody (ab77329)

ab77329 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77329.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"