Overview

  • Product name
  • Description
    Rabbit polyclonal to Meprin beta
  • Specificity
    Meprin beta antibody Math Domain recognizes meprin beta but not alpha subunits.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)(Peptide available as ab44829.)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Peptide-affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 for using colorimetric substrates such as BCIP/NBT. WB: 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 84 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Membrane metallopeptidase that sheds many membrane-bound proteins. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.
    • Tissue specificity
      The major site of expression is the brush border membrane of small intestinal and kidney epithelial cells.
    • Sequence similarities
      Belongs to the peptidase M12A family.
      Contains 1 EGF-like domain.
      Contains 1 MAM domain.
      Contains 1 MATH domain.
    • Post-translational
      modifications
      N-glycosylated; contains high mannose and/or complex biantennary structures.
      O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
      Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues.
    • Cellular localization
      Cell membrane. Secreted. Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endopeptidase 2 antibody
      • Endopeptidase-2 antibody
      • kinase splitting membranal proteinase antibody
      • KSMP antibody
      • Mep1b antibody
      • MEP1B_HUMAN antibody
      • Meprin 1 beta subunit antibody
      • Meprin A beta antibody
      • Meprin A beta subunit precursor antibody
      • Meprin A subunit beta antibody
      • Meprin B antibody
      • N benzoyl L tyrosyl P amino benzoic acid hydrolase beta subunit antibody
      • N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta antibody
      • PABA peptide hydrolase antibody
      • PPH beta antibody
      see all

    Images

    • All lanes : Anti-Meprin beta antibody (ab42743) at 1/1000 dilution

      Lane 1 : recombinant Human Meprin-alpha at 0.05 µg
      Lane 2 : negative control
      Lane 3 : recombinant Human Meprin-beta at 0.05 µg


      Predicted band size : 84 kDa
      Observed band size : 84 kDa

    References

    ab42743 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab42743.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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