Overview

  • Product nameAnti-Mer2 (phospho S30) antibody
  • Description
    Rabbit polyclonal to Mer2 (phospho S30)
  • SpecificityThis antibody reacts with MER 2 phosphorylated at Ser 30. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide: TETSSp PFRSI, corresponding to N terminal amino acids 26-35 of Saccharomyces cerevisiae Mer2

  • Positive control
    • Whole cell extract from S.cerevisiae cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/25000.
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

Target

  • RelevanceMer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • REC107 antibody
      • Meiotic recombination 2 protein antibody
      • Mer 2 antibody

    References for Anti-Mer2 (phospho S30) antibody (ab47254)

    This product has been referenced in:
    • Blitzblau HG & Hochwagen A ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. Elife 2:e00844 (2013). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24137535) »
    • Sasanuma H  et al. Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. Genes Dev 22:398-410 (2008). WB ; Saccharomyces cerevisiae . Read more (PubMed: 18245451) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. The difference is in the purification. If you look towards the bottom of the datasheet for ab47254, the purification notes say that the antibody was adsorbed against the non-phosphorylated peptide to remove antibodies re...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"