Overview

  • Product name
    Anti-Mer2 (phospho S30) antibody
  • Description
    Rabbit polyclonal to Mer2 (phospho S30)
  • Specificity
    This antibody reacts with MER 2 phosphorylated at Ser 30. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide: TETSSp PFRSI, corresponding to N terminal amino acids 26-35 of Saccharomyces cerevisiae Mer2

  • Positive control
    • Whole cell extract from S.cerevisiae cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/25000.
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

Target

  • Relevance
    Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • REC107 antibody
      • Meiotic recombination 2 protein antibody
      • Mer 2 antibody

    References for Anti-Mer2 (phospho S30) antibody (ab47254)

    This product has been referenced in:
    • Blitzblau HG & Hochwagen A ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. Elife 2:e00844 (2013). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24137535) »
    • Sasanuma H  et al. Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombination. Genes Dev 22:398-410 (2008). WB ; Saccharomyces cerevisiae . Read more (PubMed: 18245451) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your enquiry. The difference is in the purification. If you look towards the bottom of the datasheet for ab47254, the purification notes say that the antibody was adsorbed against the non-phosphorylated peptide to remove antibodies re...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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