Anti-Mesp1 antibody (ab77013)

Overview

  • Product name
  • Description
    Mouse monoclonal to Mesp1
  • Tested applications
    Suitable for: ELISA, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-63 of Human Mesp1

  • Positive control
    • FHs 173 WE cell lysate; Recombinant tagged full length human Mesp1.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab77013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function
    Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc5 antibody
    • Class C basic helix-loop-helix protein 5 antibody
    • Mesoderm posterior 1 antibody
    • Mesoderm posterior 1 homolog (mouse) antibody
    • Mesoderm posterior 1 homolog antibody
    • Mesoderm posterior protein 1 antibody
    • MESP 1 antibody
    • Mesp1 antibody
    • MESP1_HUMAN antibody
    • MGC10676 antibody
    • MGC159208 antibody
    • MGC159210 antibody
    • OTTMUSP00000030151 antibody
    see all

Images

  • Anti-Mesp1 antibody (ab77013) at 1 µg/ml + FHs 173 WE cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References

ab77013 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Mouse Cell (E7.5 embryo)
Specification
E7.5 embryo
Permeabilization
Yes - 0.1% TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 24 2014

Many thanks for contacting us last week in regards to antibodies raised against Mesp1.

As discussed over the phone, the anti-Mesp1 antibody (ab77013) has as yet not been used in ICC. This does not mean that it would not work, simply that we ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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