Overview

  • Product nameAnti-MEST antibody
    See all MEST primary antibodies
  • Description
    Goat polyclonal to MEST
  • Specificityab95453 is expected to recognize all reported isoforms.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide, corresponding to internal sequence amino acids 154-167 (

    QELLYRYKQNRSGR

    ) of Human MEST isoform 1 or amino acids 145-157 of Human MEST (Uniprot ID = Q5EB52).

  • Positive control
    • Mouse and Rat Testis lysates This antibody gave a positive result when used in the following methanol fixed cell lines: A498

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95453 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab95453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.5 µg/ml. Predicted molecular weight: 39 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificityHighly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele.
  • Sequence similaritiesBelongs to the AB hydrolase superfamily.
  • Developmental stageMonoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686L18234 antibody
    • EC 3.-.-.- antibody
    • Mesoderm specific transcript (mouse) homolog antibody
    • Mesoderm specific transcript homolog (mouse) antibody
    • Mesoderm-specific transcript homolog protein antibody
    • MEST antibody
    • MEST_HUMAN antibody
    • MGC111102 antibody
    • MGC8703 antibody
    • OTTHUMP00000210362 antibody
    • OTTHUMP00000210364 antibody
    • OTTHUMP00000210367 antibody
    • Paternally expressed gene 1 antibody
    • Paternally-expressed gene 1 protein antibody
    • PEG1 antibody
    see all

Anti-MEST antibody images

  • ICC/IF image of ab95453 stained A498 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95453 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti-goat (ab150133) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-MEST antibody (ab95453) at 0.2 µg/ml (for 1 hour) + Mouse testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa

References for Anti-MEST antibody (ab95453)

This product has been referenced in:
  • Chu DT  et al. Expression of Adipocyte Biomarkers in a Primary Cell Culture Models Reflects Preweaning Adipobiology. J Biol Chem 289:18478-18488 (2014). WB ; Mouse . Read more (PubMed: 24808178) »

See 1 Publication for this product

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Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"