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Recombinant full length protein corresponding to Human Met (c-Met). This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met
Database link: 4233
Our Abpromise guarantee covers the use of ab59884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 135 kDa (predicted molecular weight: 156 kDa).|
|IHC-P||1/50 - 1/100.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of HepG2 cells stained with ab59884 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab59884 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (ab150105) (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
ab59884 staining Met (c-Met) in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with the undiluted primary antibody. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
Immunohistochemical staining of Met (c-Met) in Human colon carcinoma tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab59884 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-mouse polyclonal (ready to use) was used as the secondary antibody.
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