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Recombinant full length protein corresponding to Human Met (c-Met). This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met
Database link: 4233
Our Abpromise guarantee covers the use of ab59884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 135 kDa (predicted molecular weight: 156 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use at an assay dependent concentration.|
Fluorescent confocal image of HepG2 cells stained with ab59884 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab59884 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (ab150105) (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
ab59884 at 1/100 dilution staining Met (c-Met) in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
ab59884 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"