The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 17455332
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionReceptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Ab27199 at a dilution of 1/50, staining metabolic glutamate recepor 1 in paraffin embedded formalin fixed human brain tissue section.
References for Anti-Metabotropic Glutamate Receptor 1 antibody (ab27199)
This product has been referenced in:
Yip D et al. A phase 0 trial of riluzole in patients with resectable stage III and IV melanoma. Clin Cancer Res15:3896-902 (2009).
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Minneci F et al. Signaling properties of stratum oriens interneurons in the hippocampus of transgenic mice expressing EGFP in a subset of somatostatin-containing cells. Hippocampus17:538-53 (2007).
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