Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211)

Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 1 antibody
    See all Metabotropic Glutamate Receptor 1 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide:

    SSVPSSPVSESVL

    , corresponding to amino acids 1159-1171 of Human Metabotropic Glutamate Receptor 1

  • Positive control
    • Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutamate receptor metabotropic 1 antibody
    • glutamate receptor, metabotropic 1 antibody
    • GPRC1A antibody
    • GRM1 antibody
    • GRM1-Alpha antibody
    • GRM1_HUMAN antibody
    • GRM1A antibody
    • Metabotropic glutamate receptor 1 antibody
    • mGlu1 antibody
    • mGluR1 antibody
    • MGLUR1-Alpha antibody
    • MGLUR1A antibody
    • SCAR13 antibody
    see all

Anti-Metabotropic Glutamate Receptor 1 antibody images

  • Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

    Secondary
    AP-conjugated secondary at 1/5000 dilution

    Predicted band size : 133 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence labeling of Metabotropic Glutamate Receptor 1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
  • Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000.
  • ab82211 staining Metabotropic Glutamate Receptor 1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References for Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211)

This product has been referenced in:
  • Valiya Veettil M  et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . Read more (PubMed: 25299066) »
  • Sylantyev S  et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . Read more (PubMed: 23395378) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample
Cat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 07 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Dr. Yuhua Liu

Verified customer

Submitted Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Rat Cell (brain)
Specification
brain
Permeabilization
Yes - 0.3% triton
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% trition
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
No
Fixative
Paraformaldehyde
Username

Wei Shi

Verified customer

Submitted Jul 16 2013

I performed a search of some other anti - metabotropic glutamate receptor 1 antibodies, and it seems the glutamate receptor can run between ˜110 - 130 kDa depending on the post-translational modification in the tested samples. Thus, the 110 kDa band th...

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