Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)


  • Product nameAnti-Metabotropic Glutamate Receptor 1a antibody
    See all Metabotropic Glutamate Receptor 1a primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 1a
  • SpecificityIHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 1159-1171 of Rat Metabotropic Glutamate Receptor 1a.

  • Positive control
    • Rat brain (singulate cortex) Human brain lysate Rat cerebellar lysates



Our Abpromise guarantee covers the use of ab51314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC (PFA fixed)
  • Application notesICC/IF: 1/250.
    Immunohistochemistry (PFA fixed): 1/250.
    WB: 1/1000. Predicted molecular weight: 133 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
    • Tissue specificityDetected in brain.
    • Involvement in diseaseSpinocerebellar ataxia, autosomal recessive, 13
    • Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Anti-Metabotropic Glutamate Receptor 1a antibody images

    • Metabotropic Glutamate Receptor 1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. Metabotropic Glutamate Receptor 1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
    • Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg

      AP-conjugated secondary at 1/5000 dilution

      Predicted band size : 133 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

      Lane 1 : Rat cerebellar membranes
      Lane 2 : Rat cerebellar membranes + DTT

      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    References for Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

    This product has been referenced in:
    • Romano C  et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). Read more (PubMed: 7636025) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Ferret Tissue lysate - whole (Parietal cortex in Brain)
    Gel Running Conditions Reduced Denaturing (Nupage 3-8% Tris-acetate gel)
    Loading amount 40 µg
    Specification Parietal cortex in Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Francis Djankpa

    Verified customer

    Submitted Jan 07 2016