Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

Overview

  • Product nameAnti-Metabotropic Glutamate Receptor 1a antibody
    See all Metabotropic Glutamate Receptor 1a primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 1a
  • SpecificityIHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    SSVPSSPVSESVL

    , corresponding to amino acids 1159-1171 of Rat Metabotropic Glutamate Receptor 1a.

  • Positive control
    • Rat brain (singulate cortex) Human brain lysate Rat cerebellar lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab51314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
IHC (PFA fixed)
  • Application notesICC/IF: 1/250.
    Immunohistochemistry (PFA fixed): 1/250.
    WB: 1/1000. Predicted molecular weight: 133 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
    • Tissue specificityDetected in brain.
    • Involvement in diseaseSpinocerebellar ataxia, autosomal recessive, 13
    • Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Anti-Metabotropic Glutamate Receptor 1a antibody images

    • Metabotropic Glutamate Receptor 1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. Metabotropic Glutamate Receptor 1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
    • Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg

      Secondary
      AP-conjugated secondary at 1/5000 dilution

      Predicted band size : 133 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

      Lane 1 : Rat cerebellar membranes
      Lane 2 : Rat cerebellar membranes + DTT


      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    References for Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

    This product has been referenced in:
    • Romano C  et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). Read more (PubMed: 7636025) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Ferret Tissue lysate - whole (Parietal cortex in Brain)
    Gel Running Conditions Reduced Denaturing (Nupage 3-8% Tris-acetate gel)
    Loading amount 40 µg
    Specification Parietal cortex in Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Francis Djankpa

    Verified customer

    Submitted Jan 07 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"