Mouse monoclonal [3A11] to Metabotropic Glutamate Receptor 2+4
Specificityab77988 recognises Metabotropic Glutamate Receptor 2 by Western blot and RIA. When using ab77988 for immunohistochemistry or immunoprecipitation it recognizes both Metabotropic Glutamate Receptor 2 and Metabotropic Glutamate Receptor 4.
ab77988, at a 1/500 dilution, staining adult monkey pryamidal cells.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Metabotropic Glutamate Receptor 2+4 antibody [3A11] (ab77988)This image was submitted by an anonymous abreviewer.
IHC-FoFr image of ab77988 staining of Metabotropic Glutamate Receptor 2+4 in rat brain cortex tissues. The animals were perfusion fixed with 4% PFA in 15% solution of saturated picric acid and 0.1M phosphate buffer. Overnight postfixation was performed with the same fixative. Brain tissue was cryoprotected in 30% sucrose solution overnight. Free-floating sections were incubated in ab77988 (1:300) for two hours at 20°C, followed by Alexa Fluor 488 conjugated secondary antibody.