Anti-Metabotropic Glutamate Receptor 2+4 antibody [3A11] (ab77988)

Overview

  • Product nameAnti-Metabotropic Glutamate Receptor 2+4 antibody [3A11]
  • Description
    Mouse monoclonal [3A11] to Metabotropic Glutamate Receptor 2+4
  • Specificityab77988 recognises Metabotropic Glutamate Receptor 2 by Western blot and RIA. When using ab77988 for immunohistochemistry or immunoprecipitation it recognizes both Metabotropic Glutamate Receptor 2 and Metabotropic Glutamate Receptor 4.
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WB, IP, ELISA, RIA, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Rat, Human, Monkey
  • Immunogen

    Recombinant fusion protein TrpE Metabotropic Glutamate Receptor 2

  • Positive control
    • Adult monkey pryamidal cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.
IHC (PFA fixed) 1/500 - 1/1000.

Target

Anti-Metabotropic Glutamate Receptor 2+4 antibody [3A11] images

  • ab77988, at a 1/500 dilution, staining adult monkey pryamidal cells.
  • IHC-FoFr image of ab77988 staining of Metabotropic Glutamate Receptor 2+4 in rat brain cortex tissues. The animals were perfusion fixed with 4% PFA in 15% solution of saturated picric acid and 0.1M phosphate buffer. Overnight postfixation was performed with the same fixative. Brain tissue was cryoprotected in 30% sucrose solution overnight. Free-floating sections were incubated in ab77988 (1:300) for two hours at 20°C, followed by Alexa Fluor 488 conjugated secondary antibody.

    See Abreview

References for Anti-Metabotropic Glutamate Receptor 2+4 antibody [3A11] (ab77988)

ab77988 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain cortex)
Specification Brain cortex
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Submitted Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"