Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088)
Key features and details
- Rabbit polyclonal to Metabotropic Glutamate Receptor 4/MGLUR4
- Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Chicken, Cat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody
See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies -
Description
Rabbit polyclonal to Metabotropic Glutamate Receptor 4/MGLUR4 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Cat, Human -
Immunogen
Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 4/MGLUR4 (C terminal).
Database link: Q14833 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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|
IHC-Fr | (5) |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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|
IHC-P | (4) |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
WB | (3) |
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
Tissue specificity
Strongly expressed in the cerebellum. Expressed at low levels in hippocampus, hypothalamus and thalamus. No expression detected in liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2914 Human
- Entrez Gene: 268934 Mouse
- Entrez Gene: 24417 Rat
- Omim: 604100 Human
- SwissProt: Q14833 Human
- SwissProt: Q68EF4 Mouse
- SwissProt: P31423 Rat
- Unigene: 654847 Human
see all -
Alternative names
- Glutamate receptor metabotropic 4 antibody
- GPRC1D antibody
- Grm4 antibody
see all
Images
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Ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088) at 1/500 dilution
Lane 1 : 293 cell extracts, treated
with Forskolin (40nM, 30mins)
Lane 2 : 293 cell extracts, treated
with Forskolin (40nM, 30mins) with peptide
Predicted band size: 102 kDa
Observed band size: 102 kDa -
ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab53088 has been referenced in 10 publications.
- Piszczek L et al. Dissociation of impulsive traits by subthalamic metabotropic glutamate receptor 4. Elife 11:N/A (2022). PubMed: 34982027
- Fisher NM et al. A GRM7 mutation associated with developmental delay reduces mGlu7 expression and produces neurological phenotypes. JCI Insight 6:N/A (2021). PubMed: 33476302
- Zhang Z et al. Activation of metabotropic glutamate receptor 4 regulates proliferation and neural differentiation in neural stem/progenitor cells of the rat subventricular zone and increases phosphatase and tensin homolog protein expression. J Neurochem 156:465-480 (2021). PubMed: 32052426
- Xiao B et al. Glutamate metabotropic receptor 4 (GRM4) inhibits cell proliferation, migration and invasion in breast cancer and is regulated by miR-328-3p and miR-370-3p. BMC Cancer 19:891 (2019). PubMed: 31492116
- Wang H et al. Expression patterns of L-amino acid receptors in the murine STC-1 enteroendocrine cell line. Cell Tissue Res 378:471-483 (2019). PubMed: 31410629
- Novati A et al. Environment-dependent striatal gene expression in the BACHD rat model for Huntington disease. Sci Rep 8:5803 (2018). PubMed: 29643462
- Jenkins BG et al. Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration. Neuropharmacology 108:462-73 (2016). PubMed: 26581500
- Chruscicka B et al. Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors. J Biomol Screen 20:350-8 (2015). PubMed: 25394730
- Betts MJ et al. Allosteric modulation of the group III mGlu receptor 4 provides functional neuroprotection in the 6-OHDA rat model of Parkinson's disease. Br J Pharmacol : (2012). PubMed: 22404342
- Wang H et al. Spinal metabotropic glutamate receptor 4 is involved in neuropathic pain. Neuroreport 22:244-8 (2011). PubMed: 21358553