Recombinant Fragment Predicted to work with:
Mouse, Rat, Horse, Chicken, Dog, Human, Chimpanzee
Recombinant fragment: CPGYAGLCDA MKPIDGRKLL ESLMKTNFTG VSGDTILFDE NGDSPGRYEI MNFKEMGKD YFDYINVGSW DNGELKMDDD EVWSKKSNII RSVCSEPCEK G, corresponding to amino acids 419-519 of Human Metabotropic Glutamate Receptor 5 (NP_000833) with a tag of 26 kDa.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 132 kDa.
Use at an assay dependent dilution. Detection limit for recombinant tagged Metabotropic Glutamate Receptor 5 is approximately 0.03ng/ml as a capture antibody.
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.