Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab76316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 132 kDa.Can be blocked with Metabotropic Glutamate Receptor 5 peptide (ab139974).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200.

Data from Abreview 42187 using rat hippocampal neurons.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutamate receptor metabotropic 5 antibody
      • GPRC1E antibody
      • Grm5 antibody
      • GRM5_HUMAN antibody
      • Metabotropic glutamate receptor 5 antibody
      • Metabotropic glutamate receptor 5 variant F antibody
      • Metabotropic glutamate receptor 5 variant G antibody
      • Metabotropic glutamate receptor 5 variant H antibody
      • mGlu5 antibody
      • mGluR5 antibody
      • PPP1R86 antibody
      • Protein phosphatase 1 regulatory subunit 86 antibody
      see all

    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] images

    • ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/2000) for 16 hours at 21°C. An alexa fluor® 594 conjugated goat anti-rabbit IgG secondary was used at 1/500.

      See Abreview

    • ab76316 staining Metabotropic Glutmate Receptor 5 in rat hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution + mouse brain lysate at 10 µg

      Secondary
      Goat anti-rabbit-HRP at 1000
      Developed using the ECL technique

      Predicted band size : 132 kDa
      Observed band size : 140-150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : >250 kDa (possible dimer).
    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.

    • ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.

      See Abreview

    • Human brain tissue with ab76316 at 1/250 dilution.

    References for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    This product has been referenced in:
    • Lee KW  et al. Alteration by p11 of mGluR5 localization regulates depression-like behaviors. Mol Psychiatry 20:1546-56 (2015). Read more (PubMed: 26370144) »
    • Golubeva AV  et al. The mouse cyclophosphamide model of bladder pain syndrome: tissue characterization, immune profiling, and relationship to metabotropic glutamate receptors. Physiol Rep 2:e00260 (2014). WB ; Mouse . Read more (PubMed: 24760514) »

    See all 5 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (COS7)
    Permeabilization Yes - 0.2%Triton
    Specification COS7
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 07 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 11 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 11 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 11 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Rat Cell (rat hippocampal neuron)
    Specification rat hippocampal neuron
    Permeabilization Yes - 0.2%Triton
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 13 2014



    I am happy to confirm that we have the suitable blocking peptide available in our catalog under the product number ab139974

    http://www.abcam.com/index.html?datasheet=139974 (or use the following: http://www.abcam.com/index.html?data...

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    Thank you for your reply.

    The blocking peptide for the Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is now available to purchase under the catalogue number ab139974. The details of this product can be viewed from the f...

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    Sorry for the delay in getting back to you.

    I have had confirmation that we do have the blocking peptide available for the rabbit monoclonal anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). If you would like for me to add ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"