The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 132 kDa.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RelevanceGlutamate receptors constitute the principal excitatory neurotransmitter
receptors in brain. Two classes of glutamate receptors exist: Ionotropic
receptors, and metabotropic receptors (mGluRs).
Metabotropic Glutamate Receptor 5b (GRM5) activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.
The metabotropic glutamate receptor type 5 (mGlu5) is expressed in two splice variants, mGlu5a and mGlu5b, which differ in that mGlu5b has a 33-amino acid insert in the intracellular C-terminal domain. This receptor subtype is highly regulated, with higher levels found in developing animals.