Anti-Metabotropic Glutamate Receptor 6 antibody - C-terminal (ab154003)

Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 6 antibody - C-terminal
    See all Metabotropic Glutamate Receptor 6 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 6 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 813-877 of Human Metabotropic Glutamate Receptor 6.

  • Positive control
    • 293T, A431, H1299 and Raji cell lysates; A431 cells; A549 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab154003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 95 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Involvement in disease
    Defects in GRM6 are the cause of congenital stationary night blindness type 1B (CSNB1B) [MIM:257270]. This disorder consits of a previously unrecognized, autosomal recessive form of congenital night blindness associated with a negative electroretinogram waveform. Patients are night blind from an early age, and when maximally dark-adapted, they could perceive lights only with an intensity equal to or slightly dimmer than that normally detected by the cone system. ERGs in response to single brief flashes of light have clearly detectable a-waves, which are derived from photoreceptors, and greatly reduced b-waves, which are derived from the second-order inner retinal neurons. ERGs in response to sawtooth flickering light indicate a markedly reduced ON response and a nearly normal OFF response. There is no subjective delay in the perception of suddenly appearing white vs black objects on a gray background.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNB1B antibody
    • DKFZp686H1993 antibody
    • GluR6 antibody
    • Glutamate receptor metabotropic 6 antibody
    • GPRC1F antibody
    • Grm6 antibody
    • GRM6_HUMAN antibody
    • Metabotropic glutamate receptor 6 antibody
    • mGlu6 antibody
    • mGluR6 antibody
    see all

Images

  • All lanes : Anti-Metabotropic Glutamate Receptor 6 antibody - C-terminal (ab154003) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 95 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling Metabotropic Glutamate Receptor 6 with ab154003 at 1/100 dilution.
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling Metabotropic Glutamate Receptor 6 with ab154003 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154003 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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