Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705)

Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 7 antibody
    See all Metabotropic Glutamate Receptor 7 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 7
  • Specificity
    ab53705 detects endogenous levels of total Metabotropic Glutamate Receptor 7 protein
  • Tested applications
    Suitable for: IP, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 7.

  • Positive control
    • Human brain tissue and HuvEc cell extracts.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function
    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Tissue specificity
    Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ40498 antibody
    • GLUR7 antibody
    • Glutamate receptor metabotropic 7 antibody
    • GPRC1G antibody
    • GRM7 antibody
    • GRM7_HUMAN antibody
    • Metabotropic glutamate receptor 7 antibody
    • MGLU7 antibody
    • mGluR7 antibody
    • OTTHUMP00000206961 antibody
    • OTTHUMP00000214674 antibody
    • OTTHUMP00000214675 antibody
    see all

Images

  • Metabotropic Glutamate Receptor 7 was immunoprecipitated using 0.5mg Rat Brain tissue extract, 5µg of Rabbit polyclonal to Metabotropic Glutamate Receptor 7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53705.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 110kDa; Metabotropic Glutamate Receptor 7

  • ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide


    Predicted band size : 102 kDa
    Observed band size : 102 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

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The immunogen sequence is from C-terminal end.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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