Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)

Overview

  • Product name
    Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin)
    See all Metallophilic Macrophages primary antibodies
  • Description
    Rat monoclonal [MOMA-1] to Metallophilic Macrophages (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Does not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
  • Tested applications
    Suitable for: IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse lymph node tissue

  • Positive control
    • Mouse spleen
  • General notes
    The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.


    Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Kathon
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
  • Clonality
    Monoclonal
  • Clone number
    MOMA-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.

    Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) images

    • ab51814 at 0.5 µg/ml staining mouse spleen; frozen tissue.

    References for Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)

    This product has been referenced in:
    • Frenkel D  et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). Read more (PubMed: 27403737) »
    • Schaefer-Klein JL  et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB . Read more (PubMed: 26560244) »

    See all 12 Publications for this product

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