Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)

Overview

  • Product nameAnti-Metallophilic Macrophages antibody [MOMA-1] (Biotin)
    See all Metallophilic Macrophages primary antibodies
  • Description
    Rat monoclonal [MOMA-1] to Metallophilic Macrophages (Biotin)
  • ConjugationBiotin
  • SpecificityDoes not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
  • Tested applicationsSuitable for: IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse lymph node tissue

  • Positive control
    • Mouse spleen
  • General notesThe antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.


    Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Kathon
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesMonoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
  • ClonalityMonoclonal
  • Clone numberMOMA-1
  • IsotypeIgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab51814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • RelevanceMetallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.

    Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) images

    • ab51814 at 0.5 µg/ml staining mouse spleen; frozen tissue.

    References for Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)

    This product has been referenced in:
    • Khan TN  et al. Prolonged apoptotic cell accumulation in germinal centers of Mer-deficient mice causes elevated B cell and CD4+ Th cell responses leading to autoantibody production. J Immunol 190:1433-46 (2013). Mouse . Read more (PubMed: 23319738) »
    • Yamazaki C  et al. Critical roles of a dendritic cell subset expressing a chemokine receptor, XCR1. J Immunol 190:6071-82 (2013). Mouse . Read more (PubMed: 23670193) »

    See all 10 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"