Anti-Metallothionein antibody (ab36882)
Key features and details
- Rabbit polyclonal to Metallothionein
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-Metallothionein antibody
See all Metallothionein primary antibodies -
Description
Rabbit polyclonal to Metallothionein -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Atlantic cod -
Immunogen
Metallothionein purified from liver of Atlantic cod (Gadus morhua).
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Positive control
- WB: Liver S9 fractions.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 1% BSA, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/50 - 1/1000. Not recommended for coating due to the BSA content.
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WB |
1/50 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).
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Notes |
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ELISA
1/50 - 1/1000. Not recommended for coating due to the BSA content. |
WB
1/50 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa). |
Target
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Function
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. -
Sequence similarities
Belongs to the metallothionein superfamily. Type 1 family. -
Domain
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. - Information by UniProt
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Alternative names
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
see all
Images
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Ab36882, at a concentration of 1 µg/ml, staining Metallothionein by ELISA. Coating buffer was used as a negative control.
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All lanes : Anti-Metallothionein antibody (ab36882) at 1/500 dilution
Lane 2 : Purified metallothionein from Atlantic cod at 0.25 µg
Lane 3 : Liver S9 fractions from cadmium treated Atlantic cod at 2.5 µg
Lane 4 : Liver S9 fractions from cadmium treated Whiting at 2.5 µg
Lane 5 : Liver S9 fractions from cadmium treated Yellow gurnard at 2.5 µg
Lane 6 : Liver S9 fractions from cadmium treated Flounder at 2.5 µg
Predicted band size: 6 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Lane 1 represents the molecular weight standard.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab36882 has been referenced in 2 publications.
- Nagamatsu PC et al. Synthetic fish metallothionein design as a potential tool for monitoring toxic metals in water. Environ Sci Pollut Res Int 28:9517-9528 (2021). PubMed: 33146826
- Yudkovski Y et al. Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Mar Environ Res 65:427-36 (2008). ELISA ; Fish . PubMed: 18342364