Overview

  • Product nameAnti-Metallothionein antibody
    See all Metallothionein primary antibodies
  • Description
    Rabbit polyclonal to Metallothionein
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Atlantic cod (Gadus morhua) and with metallothionein from a wide variety of different species including whiting (Merlangius merlangus), yellow gurnard (Trigla lucerna) and flounder (Platichthys flesus) as well as some cypriniform, siluriform, salmoniform, perciform and rajiform species.
  • Immunogen

    Metallothionein purified from liver of Atlantic cod (Gadus morhua).

  • Positive control
    • WB: Liver S9 fractions.

Properties

Applications

Our Abpromise guarantee covers the use of ab36882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50 - 1/1000. Not recommended for coating due to the BSA content.
WB 1/50 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).

Target

  • FunctionMetallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • Sequence similaritiesBelongs to the metallothionein superfamily. Type 1 family.
  • DomainClass I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
  • Information by UniProt
  • Alternative names
    • CES 1 antibody
    • CES1 antibody
    • Metallothionein 1A antibody
    • Metallothionein 1S antibody
    • Metallothionein 2 antibody
    • Metallothionein 2A antibody
    • Metallothionein IA antibody
    • Metallothionein II antibody
    • Metallothionein-1A antibody
    • Metallothionein-IA antibody
    • Metallothionein2 antibody
    • MGC32848 antibody
    • MT 1A antibody
    • MT 2 antibody
    • MT 2A antibody
    • MT IA antibody
    • MT II antibody
    • MT-1A antibody
    • MT-IA antibody
    • MT1 antibody
    • MT1A antibody
    • MT1A_HUMAN antibody
    • MT1S antibody
    • MT2 antibody
    • MT2A antibody
    • MTC antibody
    see all

Anti-Metallothionein antibody images

  • Ab36882, at a concentration of 1 µg/ml, staining Metallothionein by ELISA. Coating buffer was used as a negative control.
  • All lanes : Anti-Metallothionein antibody (ab36882) at 1/500 dilution

    Lane 2 : Purified metallothionein from Atlantic cod at 0.25 µg
    Lane 3 : Liver S9 fractions from cadmium treated Atlantic cod at 2.5 µg
    Lane 4 : Liver S9 fractions from cadmium treated Whiting at 2.5 µg
    Lane 5 : Liver S9 fractions from cadmium treated Yellow gurnard at 2.5 µg
    Lane 6 : Liver S9 fractions from cadmium treated Flounder at 2.5 µg


    Predicted band size : 6 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)

References for Anti-Metallothionein antibody (ab36882)

This product has been referenced in:
  • Yudkovski Y  et al. Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Mar Environ Res 65:427-36 (2008). ELISA ; Fish . Read more (PubMed: 18342364) »

See 1 Publication for this product

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I am very pleased to hear you would like to accept our offer and test ab12228 in zebrafish in WB. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forzebrafish in WBand include...

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Thank you very much for your interest in ab12228.

To our knowledge, ab12228 has not been tested in zebrafish in WB. Therefore, I can offer a discount off a future purchase if you buy ab12228 now, test it inzebrafish and submit feedback to us i...

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Thank you for contacting us.

Indeed, we have three metallothionein antibodies.

Please follow the links:

http://www.abcam.com/index.html?datasheet=12228 (or use the following: http://www.abcam.com/index.html?datasheet=122...

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Thank you for your enquiry. Metallothionein antibody (ab36882) is an antibody that can be applied and is guaranteed for use in ELISA. We do not sell specific kits for use with this antibody. However, the antibody can be applied relatively easily us...

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