MitoSciences (MSX32)

MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array (ab109880)

Overview

  • Product nameMetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeSandwich (quantitative)
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab109880 (MSX32) is a novel array that allows for the simultaneous quantification of 3 key enzymes involved in fatty acid oxidation: MCAD, SCHAD and Mitochondrial Trifunctional Protein. Frataxin is also included as a control.

    The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes.

    This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.

    This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 3 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.

  • Notes

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • Tested applicationsSandwich ELISAmore details
  • PlatformReagents

Properties

    Applications

    Our Abpromise guarantee covers the use of ab109880 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent dilution.

    MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array images

    • Measurement of patient and deficient cell lines. Untransformed fibroblasts cell lines cultured from patients with lipid metabolism disorders were analyzed by these dipsticks. Absorbance units were compared to neonatal skin fibroblast cells (1) as a control. Patient cell line (2) has TFP protein deficiency likely a result of mutations R61H (182G-A) and R247H (740G-A) in HADHB gene (TFP beta subunit). While cell lines (3) and (4) both have MCAD activity deficiency as a result of the prevalent MCAD mutation K304E, 985A-G) in the ACADM gene. Cell lines patients deficient in lipid metabolism were analyzed. Note the mAbs units measured for the untransformed control fibroblasts are different from the transformed HepG2 liver cell lines shown above. Also note a consistent decreased SCHAD level in these patients cell lines which may be a consequence of general lipid metabolism disruption in these patient cell lines.
    • Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies (learn more about validation) and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

    Protocols

    References for MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array (ab109880)

    ab109880 has not yet been referenced specifically in any publications.

    Product Wall

    This kit uses human specific antibodies so unfortunately this kit will not work for detecting mouse enzymes.

    I hope this is helpful. Please contact us again if you have any further questions.

    Thank you for your phone call. Unfortunately, this dipstick array has been tested on mouse samples and found to be unsuitable for that purpose. Ab109880 is only suitable for use on human samples. I hope this helps, please let me know if you need any ad...

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    Unfortunately ab109880 is suibable for use with human samples only. Please feel free to contact me if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"