Anti-Methionine Aminopeptidase 2 antibody (ab123266)

Overview

  • Product nameAnti-Methionine Aminopeptidase 2 antibody
    See all Methionine Aminopeptidase 2 primary antibodies
  • Description
    Rabbit polyclonal to Methionine Aminopeptidase 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 32-61 of Human Methionine Aminopeptidase 2 (NP_006829.1; UniProt ID: P50579), conjugated to KLH.

  • Positive control
    • K562 cell lysate and 293 cell lysate, transiently transfected with Methionine Aminopeptidase 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab123266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • FunctionCotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
    Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • Sequence similaritiesBelongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
  • Post-translational
    modifications
    Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
  • Cellular localizationCytoplasm. About 30% of expressed METAP2 associates with polysomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930035J23Rik antibody
    • AI047573 antibody
    • AL024412 antibody
    • Amp2 antibody
    • AU014659, antibody
    • EIF 2 associated p67 homolog antibody
    • EIF-2-associated p67 antibody
    • Initiation factor 2 associated 67 kDa glycoprotein antibody
    • Initiation factor 2-associated 67 kDa glycoprotein antibody
    • MAP 2 antibody
    • MAP2 antibody
    • MAP2_HUMAN antibody
    • MetAP 2 antibody
    • Metap2 antibody
    • Methionine aminopeptidase 2 antibody
    • Methionyl aminopeptidase 2 antibody
    • MGC102452 antibody
    • MGC127390 antibody
    • MGC53792 antibody
    • MNPEP antibody
    • p67 antibody
    • p67eIF2 antibody
    • Peptidase M antibody
    • Peptidase M2 antibody
    see all

Anti-Methionine Aminopeptidase 2 antibody images

  • Anti-Methionine Aminopeptidase 2 antibody (ab123266) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 53 kDa
  • All lanes : Anti-Methionine Aminopeptidase 2 antibody (ab123266) at 1/100 dilution

    Lane 1 : 293 cell lysate (non transfected)
    Lane 2 : 293 cell lysate (transiently transfected with Methionine Aminopeptidase 2)

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 53 kDa

References for Anti-Methionine Aminopeptidase 2 antibody (ab123266)

ab123266 has not yet been referenced specifically in any publications.

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