Anti-Methionine Aminopeptidase 2 antibody (ab123523)

Overview

  • Product name
    Anti-Methionine Aminopeptidase 2 antibody
    See all Methionine Aminopeptidase 2 primary antibodies
  • Description
    Rabbit polyclonal to Methionine Aminopeptidase 2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 100 to 150 of Human Methionine Aminopeptidase 2 (NP_006829.1)

  • Positive control
    • HeLa, 293T, Jurkat, NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab123523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
    Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • Sequence similarities
    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
  • Post-translational
    modifications
    Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
  • Cellular localization
    Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • A930035J23Rik antibody
    • AI047573 antibody
    • AL024412 antibody
    • Amp2 antibody
    • AU014659, antibody
    • EIF 2 associated p67 homolog antibody
    • EIF-2-associated p67 antibody
    • Initiation factor 2 associated 67 kDa glycoprotein antibody
    • Initiation factor 2-associated 67 kDa glycoprotein antibody
    • MAP 2 antibody
    • MAP2 antibody
    • MAP2_HUMAN antibody
    • MetAP 2 antibody
    • Metap2 antibody
    • Methionine aminopeptidase 2 antibody
    • Methionyl aminopeptidase 2 antibody
    • MGC102452 antibody
    • MGC127390 antibody
    • MGC53792 antibody
    • MNPEP antibody
    • p67 antibody
    • p67eIF2 antibody
    • Peptidase M antibody
    • Peptidase M2 antibody
    see all

Images

  • Detection of Methionine Aminopeptidase 2 by Western Blot of Immunprecipitate.
    Lane 1: ab123523 at 1µg/ml staining Methionine Aminopeptidase 2 in HeLa whole cell lysate immunoprecipitated using ab123523 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • All lanes : Anti-Methionine Aminopeptidase 2 antibody (ab123523) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 52 kDa


    Exposure time : 30 seconds

References

ab123523 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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